human prostate epithelial cancer cell line  (ATCC)

Journal: bioRxiv

Article Title: Salvianolic acids are natural senolytics and increase lifespan in old age

doi: 10.64898/2026.04.29.721790

Figure Lengend Snippet: ( a ) A schematic workflow illustrating the preclinical therapeutic procedure. Two weeks after subcutaneous inoculation and in vivo uptake of PC3/PSC27 recombinants, severe combined immunodeficient (M-NSG) mice were subject to either single agent or combinatorial treatment in a metronomic schedule of several cycles. The illustration was created in BioRender. ( b ) Comparative statistics of tumor end volumes. PC3 cancer cells were inoculated either alone or combined with PSC27 stromal cells before being implanted subcutaneously to the hind flank of M-NSG animals, which were administered with MIT and SAA, either alone or in combination. ( c ) Representative images of in vivo senescence in tumor foci by SA-β-gal staining. Scale bar, 50 μm. ( d ) Comparative statistics of tumor senescence as described in ( c ). ( e ) Transcriptional analysis of a subset of SASP factors expressed in epithelial vs. stromal cells acquired from tumor foci after laser capture microdissection (LCM) to isolate stromal and cancer cells, respectively. Signals were normalized to that of the sample with lowest value in the placebo group. ( f ) Transcriptional analysis of SASP factors and two canonical senescence biomarkers p16 INK4a and p21 CIP1 . ( g ) Statistical assessment of DDR and cellular apoptosis in tumors. Values are shown as the percentage of cells positively stained by IF or IHC specific to γH2AX or cleaved caspase 3 (CCL3), respectively. ( h ) Representative images of IHC staining for CCL3 at the completion of treatment regimens. Scale bar, 50 μm. ( i ) Comparative survival of animals sacrificed upon development of advanced bulky disease. Survival duration was calculated from tissue recombinant injection until death. P values were calculated by a two-sided log-rank (Mantel-Cox) test. DDR, DNA damage response. IF, immunofluorescence. IHC, immunohistochemistry. Data in b , d , e , f and g are shown as mean ± SD and representative of 3 independent biological replicates, with P values calculated by Student’s t -tests. ^, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: The human prostate epithelial cancer cell line, PC3, was from ATCC and cultured with F-12K media (10% FBS).

Techniques: In Vivo, Staining, Laser Capture Microdissection, Immunohistochemistry, Recombinant, Injection, Immunofluorescence

Journal: RSC Advances

Article Title: Clinical evaluation of [ 99m Tc]Tc-PSMA-P1: a promising SPECT radiotracer for prostate cancer imaging

doi: 10.1039/d5ra04397b

Figure Lengend Snippet: (A) cell uptake study in 22Rv1 cell line at 4 h (B) saturation binding curve of [ 99m Tc]Tc-PSMA-P1 for the determination of K d value.

Article Snippet: The 22Rv1 human prostate cancer epithelial cell line was procured from the American Type Culture Collection (ATCC).

Techniques: Binding Assay

Journal: RSC Advances

Article Title: Clinical evaluation of [ 99m Tc]Tc-PSMA-P1: a promising SPECT radiotracer for prostate cancer imaging

doi: 10.1039/d5ra04397b

Figure Lengend Snippet: Whole-body SPECT/CT imaging of [ 99m Tc]Tc-PSMA-P1 in 22Rv1 tumor-bearing mice targeting PSMA at 4 hours post-injection. [ 99m Tc]Tc-PSMA-P1 alone. (A) Blockade by co-injection of 2-PMPA (100 μg) (B).

Article Snippet: The 22Rv1 human prostate cancer epithelial cell line was procured from the American Type Culture Collection (ATCC).

Techniques: Single Photon Emission Computed Tomography, Imaging, Injection

Journal: PLOS ONE

Article Title: Autophagy and oxidative stress modulation mediate Bortezomib resistance in prostate cancer

doi: 10.1371/journal.pone.0289904

Figure Lengend Snippet: IC 50 Calculation of naïve DU-145, DU-145 RB60, and DU-145 RB60U cells. The data from crystal violet assays were analyzed using GraphPad Prism 8, and the calculated IC 50 values are presented here. The resistant cells exhibited a 5-fold increase in Bortezomib tolerance after 24 weeks of Bortezomib presence, which was augmented more following another 24 weeks of drug presence, reaching a more than 10-fold increase compared to the naïve clone. Additionally, to some extent, cross-resistance to Carfilzomib was observed; the DU-145 RB60 clone achieved 4-fold Carfilzomib resistance compared to the naïve clone after 24 weeks and an almost 6-fold change after another 24 weeks. The long-deprived clone maintained its acquired resistance to both inhibitors during the 24-week monitoring. Regarding resistance to doxorubicin, all three cell clones tested exhibited a similar response to doxorubicin, regardless of resistance to Bortezomib. The experiments were repeated in triplicate, and for the IC 50 calculation, the built-in model from GraphPad Prism 8 was used.

Article Snippet: The human prostate cancer epithelial cell line DU-145 (ATCC) was cultured in RPMI 1640 medium, supplemented with 10% Fetal Bovine Serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Clone Assay